rofiq sunaryanto - ANTIBIOTIC COMPOUND FROM MARINE ACTINOMYCETES (Streptomyces sp A11): ISOLATION AND STRUCTURE ELUCIDATON

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      Publication Details (including relevant citation   information):

        Indonesia   journal of Chemistry Vol 10, No 2 (2010)

      Abstract:

      Purification and structure elucidation of antibiotic produced by   marine actinomycetes (Streptomyces sp. A11) was   conducted. Production of antibiotic was carried out by liquid   fermentation using yeast and peptone medium for 5 days   fermentation. Purification of antibiotic was carried out by   silica gel 60 (Merck, 0.063-0.200 mm) column chromatography and   preparative HPLC. Structure elucidation was carried out using   ESI-MS, 1H NMR, 13C NMR, DEPT   13C NMR, and FTIR. This antibiotic was identified as   cyclo (tyrosyl-prolyl) /   (C14H16N2O3). This   antibiotic had biological activity to Escherichia coli  ATCC 25922, Staphylococcus aureus ATCC25923,   Bacillus subtilis ATCC 66923, Pseudomonas   aeruginosa ATCC27853, and produced by extracellular   secretion.

      Keywords: antibiotic, actinomycetes,   purification, structure elucidation

      Address (URL): http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/view/582