Publication Details (including relevant citation information):
Indonesia journal of Chemistry Vol 10, No 2 (2010)
Purification and structure elucidation of antibiotic produced by marine actinomycetes (Streptomyces sp. A11) was conducted. Production of antibiotic was carried out by liquid fermentation using yeast and peptone medium for 5 days fermentation. Purification of antibiotic was carried out by silica gel 60 (Merck, 0.063-0.200 mm) column chromatography and preparative HPLC. Structure elucidation was carried out using ESI-MS, 1H NMR, 13C NMR, DEPT 13C NMR, and FTIR. This antibiotic was identified as cyclo (tyrosyl-prolyl) / (C14H16N2O3). This antibiotic had biological activity to Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC 66923, Pseudomonas aeruginosa ATCC27853, and produced by extracellular secretion.
Keywords: antibiotic, actinomycetes, purification, structure elucidation
Address (URL): http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/view/582