rofiq sunaryanto - Cyclo (Tyrosyl-Prolyl) Produced by Streptomyces sp.: Bioactivity and Molecular Structure Elucidation

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      Journal of Microbiology Indonesia   Vol 5, No 2   (2011)


      Determination of bioactivity by minimum inhibitory concentration   (MIC) methods and molecular structure identification of   antibiotic produced by Streptomyces sp. have been   carried out. The antibiotic was produced by liquid culture using   Streptomyces sp. isolate. Purification of antibiotic was   carried out by silica gel column chromatography and preparative   HPLC. Molecular structure identification was carried out using   ESI-MS, 1H NMR, 13C NMR, and 13C   DEPT NMR. Pure antibiotic showed inhibition activity to   Gram-negative and Gram-positive bacteria. MIC to Escherichia   coli ATCC 25922, Pseudomonas aeruginosa ATCC27853 ,   Staphylococcus aureus ATCC25923, and Bacillus   subtilis ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg   mL-1 , respectively. Identification using ESI-MS   showed that the molecular weight of this antibiotic was 260 g   mol-1 , and molecular formula was   C14H16N2O3 .   Elucidation of molecular structure using 1HNMR,   13C NMR, and 13C DEPT NMRshowed that   antibiotic was cyclo(tyrosyl-prolyl).

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