rofiq sunaryanto - Diversity and Bioactivity of Marine Actinomycetes from South Coast of Yogyakarta

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  Publication Details (including relevant citation   information):

  Jurnal Pascapanen dan Bioteknologi   Kelautan dan Perikanan Vol. 7 No. 1 Tahun 2012:   3138


  Isolation, identification and cytotoxicity test of marine   actinomycetes isolated from coastal marine sediment samples taken   from south coast of Yogyakarta have been conducted. A total of 30   marine sediment samples were collected from several different   location points. Isolation was conducted using heat shock   treatment (heating at 65ºC for 60 minutes) and isolates were   grown in HV Agar medium. Active compounds were produced by   fermentation using a yeast peptone glucose medium. A number of 93   actinomycetes were isolated from 30 marine sediment sample.   Identification using 16 S rRNA showed that the most abundant   isolates were the genus of Streptomycineae 38 isolates,   followed by Micromonosporineae 17 isolates,   Corynebacterineae 16 isolates and some identified as   Micrococcineae, Streptosporangineae,   Frankineae, Propionibacterineae,   Pseudonocardineae and Proteobacteria.   Cytotoxicity assay using a cell line A549 showed that 6 isolates   exhibited strong activity against the cell line A549, 2 isolates   exhibited moderate activity, 9 isolates exhibited weak activity,   and 76 isolates exhibited no activity.

  KEYWORDS: actinomycetes, biodiversity, bioactivity, isolation

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