Publication Details (including relevant citation information):
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol. 5 No. 1, Juni 2010
Isolation, identification and cytotoxicity test of marine actinomycetes isolated from coastal marine sediment samples taken from south coast of Yogyakarta have been conducted. A total of 30 marine sediment samples were collected from several different location points. Isolation was conducted using heat shock treatment (heating at 65ºC for 60 minutes) and isolates were grown in HV Agar medium. Active compounds were produced by fermentation using a yeast peptone glucose medium. A number of 93 actinomycetes were isolated from 30 marine sediment sample. Identification using 16 S rRNA showed that the most abundant isolates were the genus of Streptomycineae 38 isolates, followed by Micromonosporineae 17 isolates, Corynebacterineae 16 isolates and some identified as Micrococcineae, Streptosporangineae, Frankineae, Propionibacterineae, Pseudonocardineae and Proteobacteria. Cytotoxicity assay using a cell line A549 showed that 6 isolates exhibited strong activity against the cell line A549, 2 isolates exhibited moderate activity, 9 isolates exhibited weak activity, and 76 isolates exhibited no activity.
KEYWORDS: actinomycetes, biodiversity, bioactivity, isolation