Rachana Bhatt - Synthesis, characterization and biodegradation of carboxymethylchitosan-g- medium chain length polyhydroxyalkanoates

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      Publication Details (including relevant citation   information):

      J.   Appl. Polym. Sci. 2008



        Grafting of medium chain length polyhydroxyalkanoates (mcl-PHA)   produced by   Comamonas testosteroni   onto carboxymethylchitosan (CMCH) using ceric ammonium nitrate   (CAN) as an initiator was carried out under nitrogen atmosphere   in aqueous medium. The grafting composition was 2 g CMCH,   0.2M    CAN, and 0.5 g mcl-PHA. The reaction was carried out at 40°C ±   1°C for 4.5 h, and reaction product was extracted by acetone   precipitation. The CMCH-g-mcl-PHA   copolymers were characterized by Fourier transform infrared   spectroscopy, Thermogravimetric analysis, differential scanning   calorimetry, and scanning electron microscopy. The data obtained   showed successful grafting of mcl-PHA onto CMCH polymer. TGA   results indicated that the graft was stable up to 380°C, and the   solubility studies revealed a high % grafting efficiency.   Biodegradation studies of the graft in terms of microbial growth,   extracellular protein concentration, and % weight loss in the   graft were carried out for 30 days using a bacterial   isolate   Burkholderia cepacia   202 and a fungal isolate   Aspergillus fumigatus   202. 93% weight loss of the graft was obtained in case of    A. fumigatus   202, whereas   B. cepacia   202 showed 76% loss in weight of the graft.

      Address (URL): http://onlinelibrary.wiley.com/doi/10.1002/app.28678/abstract