Triet Truong - New Primers Reveal the Presence of a Duplicate Histone H3 in the Marine Turtle Leech Ozobranchus branchiatus

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  Publication Details (including relevant citation   information):

    P. Lavretsky, T. M. Truong, Audrey E. McGowin, G. H. Balazs, and   Jeffrey L. Peters. "New Primers Reveal the Presence of a   Duplicate Histone H3 in the Marine Turtle Leech   Ozobranchus branchiatus"    Conservation Genetics Resources   4.2 (2012): 487-490.


    Marine leeches, specific to sea turtles, have been implicated as   potential vector organisms in the spread of fibropapillomatosis   (FP), a pandemic neoplastic disease with Chelonia mydas having   the highest affliction rate. Polymerase chain reaction identified   two independent, seemingly functional histone H3 loci for marine   turtle leeches Ozobranchus branchiatus collected from C. mydas in   Florida and Hawaii. Primers were developed to amplify each   product separately. Among the two loci, sequence differentiation   (UST) ranged from 0.161 to 0.182 with identical amino acid   translations among the 22 samples. A maximum parsimony tree of   GenBank histone H3 sequences from annelids indicated the gene   duplication occurred within the Ozobranchidae family.   Geographically separated populations yielded UST values of   0.004–0.005 but were phylogenetically distinctive. These novel   markers will be useful in identifying ectoparasites in FP   research, evaluating other histone variants, and chromatin   dynamics regulation studies.

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