Abhiram Arunkumar - Fractionation of alpha-lactalbumin and beta-lactoglobulin from bovine milk serum using staged positively charged tangential flow ultrafiltration membranes

Document created by Abhiram Arunkumar on Aug 22, 2014
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  Publication Details (including relevant citation   information):

  A. Arunkumar, M.R.Etzel (2014), J. Membr. Sci., 454, 488


  1.         Fractionation of bovine a-lactalbumin     (ALA) from b-lactoglobulin     (BLG) in
        milk serum permeate (MSP) using tangential flow ultrafiltration     was examined in
        this work. By placing a positive charge on a 300 kDa     regenerated cellulose
        ultrafiltration membrane, it was possible to increase the     selectivity for
        fractionating ALA and BLG by 180 % compared to an uncharged     membrane. Thus,
        like-sized proteins that differed only somewhat in isoelectric     point, and that
        were about 15-20 times smaller than the membrane molecular     weight cutoff, were
        fractionated using charged ultrafiltration membranes.     Electrostatic repulsion
        was solely responsible for the improved selectivity. Protein     sieving
        coefficients were explained by the net charge on the protein     and the stagnant
        film model. From experimental data using a single-stage system     under total
        recycle conditions, mass balance models were used successfully     to predict the
        performance of a multi-stage membrane system. A three-stage     system gave 87 %
        pure ALA and 83 % pure BLG. Thus, dairy protein fractions of     chromatographic
        purity can be obtained using charged ultrafiltration membranes     arranged in
        stages, without the wastewater generation and expense of     chromatography. The
        results of this study will be useful for expanding the utility     of
        ultrafiltration membranes beyond simple protein concentration     to fractionation
        of proteins.

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