Jeremy Steinbacher - Adsorption and Release of siRNA from Porous Silica

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      Publication Details (including relevant citation   information): Steinbacher, Jeremy L., Landry,   Christopher C., Langmuir, 2013

      Abstract: Porous silica particles are potential   transfection agents for nucleic acid-based therapies because of   their large specific surface areas and pore volumes and the ease   with which they can be chemically modified to maximize the   loading of cargo and to effect targeting in vivo. Here, we   present a systematic study of the effects of pore size and pore   modification on the adsorption and release of short, interfering   RNA (siRNA) from a mesoporous silica particle developed in our   laboratory. Using adsorption isotherms and release experiments,   we found that the short polyamine diethylenetriamine was the best   chemical modification for achieving both the adsorption and   release of large amounts of siRNA. The degree of   functionalization with diethylenetriamine caused drastic changes   in the loading capacity and binding strength of siRNA to silica   with relatively large pores (8 nm and larger), but the degree of   functionalization had a weaker effect in narrow pores (4 nm).   Multilayer adsorption could occur in materials with large pores   (15 nm). Release experiments showed that intermediate pore sizes   and intermediate degrees of functionalization resulted in the   best compromise between maximizing loading (from strong   adsorption) and maximizing release. Capillary electrophoresis and   quantitative, real-time PCR demonstrated that siRNA was released   intact and that these particles functioned as a transfection   agent of mammalian cells in vitro.

      Address (URL): http://dx.doi.org/10.1021/la402850m