Samantha Desmarais - Isolation and preparation of bacterial cell walls for Compositional Analysis by Ultra Performance Liquid Chromatography

Document created by Samantha Desmarais on Aug 22, 2014
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  The   bacterial cell wall is composed of peptidoglycan, a   macromolecular network of sugar strands crosslinked by peptides.   Ultra Performance Liquid Chromatography provides high resolution   and throughput for novel discoveries of peptidoglycan   composition. We present a procedure for the isolation of cell   walls (sacculi) and their subsequent preparation for analysis via   UPLC. 2014 J.   Vis. Exp. Jan 15; (83).

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