Samantha Desmarais - Quantitative Experimental Determination of Primer-Dimer Formation Risk by Free-Solution Conjugate Electrophoresis

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  Publication Details (including relevant citation   information):

  To   gain better understanding of biophysical parameters constraining   primer–dimer

  formation   between primers that incorporate barcode sequences, I developed   a

  capillary   electrophoresis method that utilizes drag-tag-DNA conjugates to   quantify

  dimerization   risk between primer-barcode pairs. Results obtained with this   unique freesolution

  conjugate   electrophoresis approach are useful as quantitatively precise   input

  data   to parameterize computation models of dimerization risk. The use   of

  free-solution   electrophoresis in combination with a peptoid drag-tag and   different

  fluorophores   enabled precise separation of short DNA fragments to establish a   new

  mobility   shift assay for detection of primer–dimer formation.

    Electrophoresis 2012,33:   483–491.


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