Publication Details (including relevant citation information):
To gain better understanding of biophysical parameters constraining primer–dimer
formation between primers that incorporate barcode sequences, I developed a
capillary electrophoresis method that utilizes drag-tag-DNA conjugates to quantify
dimerization risk between primer-barcode pairs. Results obtained with this unique freesolution
conjugate electrophoresis approach are useful as quantitatively precise input
data to parameterize computation models of dimerization risk. The use of
free-solution electrophoresis in combination with a peptoid drag-tag and different
fluorophores enabled precise separation of short DNA fragments to establish a new
mobility shift assay for detection of primer–dimer formation.
Electrophoresis 2012,33: 483–491.
Address (URL): http://