Publication Details (including relevant citation information):
Society of Environmental Toxicology and Chemistry
North America 34th Annual Meeting
Harmonizing Science Across Disciplines
17–21 November 2013
Chiral human pharmaceuticals including atenolol, fluoxetine, ibuprofen and tramadol are environmental pollutants of emerging concern. Normal-phase and reversed-phase chiral liquid chromatography (HPLC-UV) methods were developed to resolve stereoisomers of atenolol, fluoxetine, ibuprofen and tramadol. In normal phase, enantiomeric resolution (RS) of tramadol was 7.95 and ibuprofen was 1.66, obtained using Chiralpak AD-H and Chiralcel OJ columns, respectively. The mobile phases were n-hexane/2-propanol/diethylamine (90:10:0.1, v/v/v) and n-hexane/2-propanol/trifluoroacetic acid (98:2:0.1, v/v/v) for the enantioseparation of tramadol and ibuprofen, respectively. Enantiomers of atenolol and fluoxetine were resolved using Chirobiotic V column and a mobile phase constituting methanol, 15 mM ammonium acetate and 0.005% formic acid. The RS of atenolol and fluoxetine were 2.40 and 2.24, respectively. The methods were subsequently used to evaluate changes in enantiomeric composition of these compounds in river water and wastewater, as well as sediments. Results from these experiments will be discussed at the meeting.