Edmond Sanganyado - Enantioselective analysis of chiral pharmaceuticals in wastewater and  environmental water samples

Version 1

      Publication Details (including relevant citation   information):

      Society of Environmental Toxicology and Chemistry

      North America 34th Annual Meeting

      Harmonizing Science Across Disciplines

      Nashville, Tennessee

      17–21 November 2013


      Chiral human pharmaceuticals including atenolol, fluoxetine,   ibuprofen and tramadol are environmental pollutants of emerging   concern. Normal-phase and reversed-phase chiral liquid   chromatography (HPLC-UV) methods were developed to resolve   stereoisomers of atenolol, fluoxetine, ibuprofen and tramadol. In   normal phase, enantiomeric resolution (RS) of tramadol was 7.95   and ibuprofen was 1.66, obtained using Chiralpak AD-H and   Chiralcel OJ columns, respectively. The mobile phases were   n-hexane/2-propanol/diethylamine (90:10:0.1, v/v/v) and   n-hexane/2-propanol/trifluoroacetic acid (98:2:0.1, v/v/v) for   the enantioseparation of tramadol and ibuprofen, respectively.   Enantiomers of atenolol and fluoxetine were resolved using   Chirobiotic V column and a mobile phase constituting methanol, 15   mM ammonium acetate and 0.005% formic acid. The RS of   atenolol and fluoxetine were 2.40 and 2.24, respectively. The   methods were subsequently used to evaluate changes in   enantiomeric composition of these compounds in river water and   wastewater, as well as sediments. Results from these experiments   will be discussed at the meeting.

      Address (URL): http://http://www.environmentalhealthnews.org/ehs/news/2013/pdf-links/SETAC-Nash ville-abstract-book-1.pdf