Molla Islam - Label-free detection of low protein concentration in solution using a novel colorimetric assay.

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      Publication Details (including relevant citation   information): Islam, Molla R., Serpe, Michael J.,   Biosens. Bioelectron., 2013,   49, pp 133-138

      Abstract: Dual pH and temp. sensitive   microgel-based etalons were fabricated by sandwiching a   "monolithic" microgel layer between two semitransparent, Au   layers. The devices exhibit visual color and multipeak   reflectance spectra, both of which primarily depend on the   distance between the Au surfaces mediated by the microgel diam.   We found that a polycationic polyelectrolyte can penetrate   through the Au overlayer to interact with neg. charged microgel   confined between Au overlayers. In this submission we report that   biotinylated polycationic polymer can penetrate through the Au   overlayer of a poly (N-isopropylacrylamide)-co-acrylic acid   (pNIPAm-co-AAc) microgel-based etalon and cause the microgel   layer to collapse. The collapse results in a shift in the   spectral peaks of the reflectance spectra. We found that the   extent of peak shift depends on the amt. of biotinylated   polycation added to the etalon, which can subsequently be used to   det. the concn. of streptavidin in soln. at pM concns. [on   SciFinder(R)]

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