Matteo Castronovo - Quantitative Study of the Effect of Coverage on the Hybridization Efficiency of Surface-Bound DNA Nanostructures

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      Publication Details (including relevant citation   information): Mirmomtaz, Elham, Castronovo, Matteo,   Grunwald, Christian, Bano, Fouzia, Scaini, Denis, Ensafi, Ali A.,   Scoles, Giacinto, Casalis, Loredana, 2008,   8 (12), pp 4134-4139

      Abstract: We demonstrate that, contrary to   current understanding, the density of probe molecules is not   responsible for the lack of hybridization in high density   single-stranded DNA (ss-DNA) self-assembled monolayers (SAMs). To   this end, we use nanografting to fabricate well packed ss-DNA   nanopatches within a ?carpet matrix? SAM of inert thiols on gold   surfaces. The DNA surface density is varied by changing the   ?writing? parameters, for example, tip speed, and number of scan   lines. Since ss-DNA is 50 times more flexible than ds-DNA,   hybridization leads to a transition to a ?standing up? phase.   Therefore, accurate height and compressibility measurements of   the nanopatches before and after hybridization allow reliable,   sensitive, and label-free detection of hybridization.   Side-by-side comparison of self-assembled and nanografted   DNA-monolayers shows that the latter, while denser than the   former, display higher hybridization efficiencies.

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