Tobias Rogosch - Differences in the composition of the human antibody repertoire by B cell subsets in the blood.

Version 1

      Publication Details (including relevant citation   information):

      Mroczek ES, Ippolito GC, Rogosch T, Hoi KH, Hwangpo TA, Brand MG,   Zhuang Y, Liu CR, Schneider DA, Zemlin M, Brown EE, Georgiou G,   Schroeder HW Jr.

      Front   Immunol. 2014 Mar 19;5:96.

      doi: 10.3389/fimmu.2014.00096

      Abstract:

      The vast initial diversity of the antibody repertoire is   generated centrally by means of a complex series of V(D)J gene   rearrangement events, variation in the site of gene segment   joining, and TdT catalyzed N-region addition. Although the   diversity is great, close inspection has revealed distinct and   unique characteristics in the antibody repertoires expressed by   different B cell developmental subsets. In order to illustrate   our approach to repertoire analysis, we present an in-depth   comparison of V(D)J gene usage, hydrophobicity, length, DH   reading frame, and amino acid usage between heavy chain   repertoires expressed by immature, transitional, mature, memory   IgD(+), memory IgD(-), and plasmacytes isolated from the blood of   a single individual. Our results support the view that in both   human and mouse, the H chain repertoires expressed by individual,   developmental B cell subsets appear to differ in sequence   content. Sequencing of unsorted B cells from the blood is thus   likely to yield an incomplete or compressed view of what is   actually happening in the immune response of the individual. Our   findings support the view that studies designed to correlate   repertoire expression with diseases of immune function will   likely require deep sequencing of B cells sorted by subset.

      Address (URL): http://www.ncbi.nlm.nih.gov/pubmed/24678310