Allison Cockrell - Changing Iron Content of the Mouse Brain During Development

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      Gregory P. Holmes Hampton,   Mrinmoy Chakrabarti, Allison L. Cockrell, Sean   P. McCormick, Louise C. Abbott, Lora S. Lindahl, and Paul A.   Lindahl (2012) Metallomics 4, 761 – 770


        Iron is crucial to many processes in the brain yet the   percentages of the major iron-containing species contained   therein, and how these percentages change during development,   have not been reliably determined. To do this, C57BL/6 mice were   enriched in 57Fe   and their brains were examined by   Mössbauer, EPR,   and electronic absorption spectroscopy;   Fe concentrations were evaluated   using ICP-MS.   Excluding the contribution of residual   blood hemoglobin,   the three major categories of brain Fe included ferritin (an iron   storage protein),   mitochondrial iron (consisting primarily of Fe/S clusters   and hemes),   and mononuclear nonheme high-spin (NHHS) FeII and   FeIII species.   Brains from prenatal and one-week old mice were dominated by   ferritin and were deficient in mitochondrial Fe. During the next   few weeks of life, the brain grew and experienced a burst of   mitochondriogenesis. Overall brain Fe concentration and the   concentration of ferritin declined during this burst phase,   suggesting that the rate of Fe incorporation was insufficient to   accommodate these changes. The slow rate of Fe import and export   to/from the brain, relative to other organs, was verified by an   isotopic labeling study. Iron levels and ferritin stores   replenished in young adult mice. NHHS FeII species   were observed in substantial levels in brains of several ages. A   stable free-radical species that increased with age was observed   by EPR spectroscopy.   Brains from mice raised on an Fe-deficient diet showed depleted   ferritin iron but normal mitochondrial iron levels.

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