Emiko Okamura - Uptake of Sevoflurane Limited by the Presence of Cholesterol in the Lipid Bilayer Membrane: A Multinuclear Nuclear Magnetic Resonance Study

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      Publication Details (including relevant citation   information):

         E. Okamura, Y. Takechi, and K. Aki, J. Oleo Sci.,63, 1149-1157 (2014).    


      The effect of   cholesterol on the uptake of a fluorinated general anesthetic,   sevoflurane (SF, fluoromethyl   2,2,2-trifluoro-1-[trifluoromethyl]ethyl ether) was studied by   multinuclear, high-resolution nuclear magnetic resonance (NMR)   spectroscopy in combination with a pulsed-field gradient   technique. Using large unilamellar vesicles of egg   phosphatidylcholine/egg phosphatidylglycerol/cholesterol as model   fluid cell membranes, the 19F and 1H NMR   chemical shifts, longitudinal relaxation times   (T1), and diffusion coefficients   (Deff) were systematically analyzed to   quantify the modulation of SF uptake to the lipid membrane by   cholesterol. All NMR parameters (chemical shift,   T1, and Deff) showed that   SF uptake is limited by the presence of cholesterol in the   membrane. SF uptake at 40 mol% cholesterol was limited to 50%–60%   of the partitioning fraction in the absence of cholesterol in the   membrane. This finding is attributed to the loss of motional   freedom in the rigid membrane environment, as demonstrated by the   gradual slowdown of lipid mobility Deff with   increase in cholesterol concentration from 0 mol% to 40   mol%.

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