Megan Maurer - Comparative Proteomic and Metabolomic Studies of Pulmonary Nanoparticle Exposure in Rats using Liquid Chromatography Tandem Mass Spectrometry

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  Publication Details (including relevant citation   information):

  Maurer, MM; Donohoe, GC; Maleki, H; Yi, J; McBride, C;   Nurkiewicz,TR; Valentine,SJ. "Comparative Proteomic and   Metabolomic Studies of Pulmonary Nanoparticle Exposure in Rats   using Liquid Chromatography Tandem Mass Spectrometry." Poster,   American Society for Mass Spectrometry Conference, St. Louis, MO,   June 2015.


  Mounting evidence suggests that pulmonary exposure to   nanoparticles (NPs) has a toxic effect on biological   systems.  A number of studies have shown that exposure to   NPs result in systemic inflammatory response, oxidative stress,   and leukocyte adhesion. However, significant knowledge gaps exist   for understanding the key molecular mechanisms responsible for   altered microvasculature function. Utilizing comprehensive liquid   chromatography tandem mass spectrometry (LC-MS/MS) and   comparative proteomic analysis strategies, important proteins   related to TiO2 NP exposure in rat plasma have been   identified. Molecular pathway analysis of these proteins revealed   the 2 canonical   pathways as being down regulated (acute phase response signaling   and LXR/RXR activation), and 1 canonical pathway as being up   regulated (production of nitric oxide and reactive oxygen species   in macrophages).   This work lays the foundation   for future research that will monitor protein dysregulation as a   function of post-exposure time and TiO2 NP dosage to   further elucidate mechanisms of pathway activation as well as to   decipher other affected pathways.

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