Publication Details (including relevant citation information):
Maurer, MM; Donohoe, GC; Maleki, H; Yi, J; McBride, C; Nurkiewicz,TR; Valentine,SJ. "Comparative Proteomic and Metabolomic Studies of Pulmonary Nanoparticle Exposure in Rats using Liquid Chromatography Tandem Mass Spectrometry." Poster, American Society for Mass Spectrometry Conference, St. Louis, MO, June 2015.
Mounting evidence suggests that pulmonary exposure to nanoparticles (NPs) has a toxic effect on biological systems. A number of studies have shown that exposure to NPs result in systemic inflammatory response, oxidative stress, and leukocyte adhesion. However, significant knowledge gaps exist for understanding the key molecular mechanisms responsible for altered microvasculature function. Utilizing comprehensive liquid chromatography tandem mass spectrometry (LC-MS/MS) and comparative proteomic analysis strategies, important proteins related to TiO2 NP exposure in rat plasma have been identified. Molecular pathway analysis of these proteins revealed the 2 canonical pathways as being down regulated (acute phase response signaling and LXR/RXR activation), and 1 canonical pathway as being up regulated (production of nitric oxide and reactive oxygen species in macrophages). This work lays the foundation for future research that will monitor protein dysregulation as a function of post-exposure time and TiO2 NP dosage to further elucidate mechanisms of pathway activation as well as to decipher other affected pathways.