Emiko Okamura - Lateral Diffusion of Lipids Separated from Rotational and Translational Diffusion of a Fluid Large Unilamellar Vesicle

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      Publication Details (including relevant citation   information):

         N. Yoshii, T. Emoto, E. Okamura,Colloid Surf. B-Biointerfaces, 106, 22-27 (2013).    

      Abstract:

      A new method to   separate lateral diffusion   of lipids in spherical  large unilamellar   vesicles from the rotational and the translational diffusion of   the vesicle as a whole is   proposed. The lateral   diffusion coefficient DL is obtained as a   time-dependent part of the observed diffusion coefficient in   vesicles of 800-nm diameters, by systematically changing the   diffusion time interval of the high-field-gradient NMR   measurement. Although the lipid is in a confined space, the   DL of 1,2-dipalmitoyl-sn-glycero-3-phosphocholineis  (1.5±0.6)´10-11  m2 s-1 in the fluid   state at  45  ˚C, more than one   order of magnitude faster than the rotational and the   translational diffusion coefficients of the vesicle by the   hydrodynamic continuum model. The method provides a potential for   quantifying the lateral diffusion of lipids and proteins in   fluid bilayer vesicles   as model cell membranes in a natural   manner.

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