Emiko Okamura - Slow Tumbling but Large Protrusion of Phospholipids in the Cell Sized Giant Vesicle

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      Publication Details (including relevant citation   information):

         Y. Takechi, H. Saito, E. Okamura,Chem. Phys. Lett.570, 136-140 (2013).    


        Lipid membrane   dynamics in cell sized vesicles (CSVs) in water is characterized   by the solution-state  NMR.  CSV is   successfully prepared by natural swelling method using osmotic   effect. Broad   1H  NMR signal and   large 31P NMR  chemical shift   anisotropy show the slow   tumbling of the lipid   with the rotational   correlation time 0.2-2.8  s in CSV  of   10-20 mm-diameters. The   1H-1H nuclear Overhauser effect  enhancement  reveals the   proximity between the lipid headgroup and alkyl chains, in which   the close  distance,~0.4 nm.  The proximity is   due to the large   protrusion of the lipids in   the vertical direction to the CSV  surface.

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