Emiko Okamura - Regulation of Phospholipid Protrusion in the Cell Sized Vesicle by Hydrophobic Bisphenol A

Document created by Emiko Okamura on May 28, 2015Last modified by Emiko Okamura on Apr 14, 2016
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  Publication Details (including relevant citation   information):

     Y. Takechi,   Y. Shintani, D. Kimoto, E. Okamura,   Membrane,40, 38-45 (2015).      


  The regulation of   the phospholipid   protrusion, the vertical   fluctuation at the surface of cell-sized vesicles (CSVs), was   investigated by solution NMR in combination with the   1H-1H nuclear Overhauser effect (NOE)   enhancement measurement. Using deuterated   bisphenol A (BP-d) and palmitic acid (PA-d) as model hydrophobic   components, we compared how the lipid protrusion is modulated by   the presence of BP-d and PA-d in the fluid, soft CSV   membrane.   It is found that the phospholipid protrusion is suppressed by   BP-d but not by PA-d, although both BP-d and PA-d are trapped in   the membrane inside. The contrasting effect of BP-d and PA-d on   the protrusion motion is interpreted by the difference in the   mode of interaction between these substances and lipids in the   membrane; rather hydrogen bonding than van der Waals forces is   the decisive parameter to regulate the phospholipid protrusion   motion.

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