Megan Maurer - Comparative plasma proteomic studies of pulmonary TiO2 nanoparticle exposure in rats using liquid chromatography tandem mass spectrometry

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  Publication Details (including relevant citation   information):

    Maurer, Megan M., Gregory C. Donohoe, Hossein Maleki, Jinghai Yi,   Carroll McBride, Timothy R. Nurkiewicz, and Stephen J. Valentine.   "Comparative plasma proteomic studies of pulmonary TiO 2   nanoparticle exposure in rats using liquid chromatography tandem   mass spectrometry." Journal   of Proteomics (2015). 



  Mounting evidence suggests that pulmonary exposure to   nanoparticles (NPs) has a toxic effect on biological systems. A   number of studies have shown that exposure to NPs result in   systemic inflammatory response, oxidative stress, and leukocyte   adhesion. However, significant knowledge gaps exist for   understanding the key molecular mechanisms responsible for   altered microvasculature function. Utilizing comprehensive   LC-MS/MS and comparative proteomic analysis strategies, important   proteins related to TiO2 NP exposure in rat plasma have been   identified. Molecular pathway analysis of these proteins revealed   13 canonical pathways as being significant (p ≤ 0.05), but none   were found to be significantly up or down-regulated (z > |2|).   This work lays the foundation for future research that will   monitor relative changes in protein abundance in plasma and   tissue as a function of post-exposure time and TiO2 NP dosage to   further elucidate mechanisms of pathway activation as well as to   decipher other affected pathways.

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