Uttam Pal - Binding of hemoglobin to ultrafine carbon nanoparticles: a spectroscopic insight into a major health hazard

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  Publication Details (including relevant citation   information):

  Banerji, Biswadip, Pramanik, Sumit Kumar, Pal, Uttam, Maiti,   Nakul Chandra 4 (43) 22536-22541

  Abstract: Carbon nanoparticles (CNPs) are light   and easily absorb into different parts of organs of the human   body. They are suspended particulate matters of respirable sizes.   In the atmosphere, ultrafine CNPs are known to be generated   mainly from the combustion of fuels and have been reported to be   a major contributor to the induction of cardiopulmonary diseases.   In third world countries, these diseases are more prevalent   because of the higher abundance of ultrafine CNPs in the air.   Different nanostructured materials, when exposed to the human   body, can easily enter into the body through the lungs or other   organs and tissues. In the laboratory, ultrafine carbon   nanoparticles were synthesized and their structure was confirmed   by DLS experiments, TEM and AFM imaging studies. Their   interactions with hemoglobin (Hb) and myoglobin (Mb) were studied   using fluorescence spectroscopy. The results indicate a   remarkably strong interaction between carbon nanoparticles and Hb   (or Mb). Temperature dependent steady state fluorescence   spectroscopy showed exothermic binding of Hb to CNPs, which is   favored by enthalpy and entropy changes. A circular dichroism   study also indicated significant change in the protein secondary   structure and a partial unfolding of the helical conformation.   These findings are highly important for understanding the   interactions between CNPs and Hb (or Mb), which might help to   better clarify the potential risks and undesirable health hazards   associated with carbon nanoparticles.

  Address (URL): http://pubs.rsc.org/en/content/articlelanding/2014/ra/c4ra02569e

 

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