Giovana Celli - Optimization of ultrasound-assisted extraction of anthocyanins from lowbush blueberries (Vaccinium angustifolium Aiton)

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  Publication Details (including relevant citation   information):

  Authors: Rojin Dibazar, Giovana B. Celli, Marianne Su-Ling Brooks   & Amyl Ghanem

  Journal of Berry Research, vol. 5, pp. 173-181


  BACKGROUND: A considerable body of evidence has associated the   consumption of blueberries to health-related benefits, mainly   because of their anthocyanin content. The extraction of these   compounds could contribute to their application in functional   foods and value-added products.

  OBJECTIVE: In this study, we investigated the ultrasound-assisted   extraction (UAE) of anthocyanins from lowbush blueberries in a   bench-scale system.

  METHODS: Two statistical design methods, namely full factorial   and Box-Behnken, were used for the screening and optimization of   the variables that significantly affect the UAE of anthocyanins.   Extraction temperature, time, solvent concentration (acidified   ethanol), and solvent to solid ratio were selected to determine   higher anthocyanin extraction (assessed by the pH-differential   method).

  RESULTS: When evaluated by response surface methodology, solvent   to solid ratio and solvent concentration had a significant effect   on UAE followed by ultrasound bath temperature. The mathematical   model indicated that the highest anthocyanin extraction would be   obtained with 60% acidified ethanol, solvent to solid ratio of   50 mL/g, at 65°C for 11.5 min.

  CONCLUSION: Ultrasound-assisted extraction was shown to be an   effective method of extracting total anthocyanins from Nova   Scotia lowbush blueberries. A statistical model to predict   optimum conditions for extraction was developed using a Box   Behnken design.

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