Giovana Celli - Optimized encapsulation of anthocyanin-rich extract from haskap berries (Lonicera caerulea L.) in calcium-alginate microparticles

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      Publication Details (including relevant citation   information):

      Authors: Giovana B. Celli, Amyl Ghanem & Marianne Su-Ling   Brooks

      Journal of Berry Research, DOI 10.3233/JBR-150107


      BACKGROUND: The chemical instability of extracted anthocyanins   (ACNs) limits their application and broader use as food colorants   and health-promoting functional ingredients. Encapsulation   technology can improve ACN stability and widen their potential   applications. 

      OBJECTIVE: The objective of this study was to optimize the   microencapsulation of ACNs from haskap berries (Lonicera caerulea   L.) in calcium-alginate particles by the extrusion/gelation   method.

      METHODS: Response Surface Methodology (RSM) by Box-Behnken (BB)   design was used for the optimization, followed by the   desirability function. Three input variables were evaluated:   concentrations of sodium alginate (x1, w/w %) and calcium   chloride (x2, w/v %), and gelation time (x3, min). The responses   were encapsulation efficiency (y1, %) and particle size (y2,   μm). 

      RESULTS: There was a good fit for the model where encapsulation   efficiency was used as a separate response (R2 = 97.98%),   however, the model for particle size did not give as good an   agreement (R2 = 63.86%). The desirability function was used to   optimize the two responses simultaneously and the optimum   conditions were determined as 9.0% (w/w) alginate solution, 2.0%   (w/v) CaCl2, and 10 min in the gelation solution. 

      CONCLUSIONS: These results illustrate the application of RSM   followed by a desirability function to optimize encapsulation   parameters for a combined response, where several measures are   considered.

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