Giovana Celli - Optimization of Ultrasound-Assisted Extraction of Anthocyanins from Haskap Berries (Lonicera caerulea L.) using Response Surface Methodology

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  Authors: Giovana B. Celli, Amyl Ghanem & Marianne Su-Ling   Brooks

  Ultrasonics Sonochemistry, vol. 27, pp. 449-455


  Haskap berries (Lonicera caerulea L.) are a rich source of   bioactive molecules. As such, the extraction of anthocyanins is   important for the development of many value-added products and   functional food ingredients. In this paper, the   ultrasound-assisted extraction (UAE) of anthocyanins from haskap   berries was investigated. Significant independent variables were   screened and optimized using Plackett–Burman (PB) and Box–Behnken   (BB) designs, respectively. The mathematical model showed a high   coefficient of determination (R2 = 0.9396) and the optimum   conditions for the extraction were as follows: liquid/solid ratio   25:1 (mL/g), solvent composition of 80% ethanol, addition of 0.5%   formic acid, ultrasound bath tempera- ture of 35 °C for 20 min.   Under these conditions, the total anthocyanin content of 22.73 mg   cyaniding 3-glucoside equivalents (C3G)/g dry weight (DW) was   consistent with the predicted response of 22.45 mg C3G/g DW from   the model (mean error of 1.28%). Five anthocyanins were   identified in the optimized extract, namely cyanidin   3,5-diglucoside, cyanidin 3-glucoside, cyanidin 3-rutinoside,   pelargonidin 3-glucoside, and peonidin 3-glucoside. Thus, UAE is   a suitable technique for the extraction of anthocyanins from   haskap berries.

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