Emiko Okamura - Kinetics of Membrane Binding and Mobility of Drugs by Multinuclear Dynamic NMR in Situ

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      Publication Details (including relevant citation   information):

      Emiko Okamura, Journal of the Society of Japanese Women   Scientists, 16, 1-8  (2016),


      Great attention has been paid to molecular dynamics of drug   deliveries to lipid bilayer membranes

      as a primary stage of bioactivities in the cell. Lipid bilayer   membranes are dynamic structures where

      molecules are moving and fluctuating under physiological   conditions. Thus the mechanisms of drug deliveries

      are considered to be relevant to such dynamics in soft, fluid   membrane interface. To gain insight into molecular

      motions and fluctuations in membranes, methods are critical   factors to be developed. In this review, a

      noninvasive strategy to monitor dynamic properties of drugs and   lipid membranes is introduced. By applying

      multinuclear high-resolution solution NMR in combination with the   pulsed-field-gradient (PFG) technique,

      dynamic aspects of drug deliveries have been quantified without   undesirable perturbation of the system. Special

      focus is on how much and how fast drugs are bound to the   membrane, and how fast drugs are moving

      within the membrane in situ by using lipid bilayer vesicles as   model cell membranes. An anticancer 5-fluorouracil,

      a fluorinated derivative of an endocrine disruptor, bisphenol A,   a neuropeptide enkephalin, and an anesthetic

      sevoflurane are compared as a model drug. The effect of   cholesterol on deliveries is also discussed

      in relation to the dynamics in membranes.

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