Elancheran Ramakrishnan - Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides Lam. by UPLC-ESI-MS/MS.

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      Publication Details (including relevant citation   information):

        Food   Chem. 2016   Jul 15;203:521-9. doi: 10.1016/j.foodchem.2016.02.101. Epub 2016   Feb 15.


        The aim of the study was to identify the phenolic compounds   present in Hydrocotyle sibthorpioides (HS), Centella asiatica   (CA) and Amaranthus viridis (AV) extracts and investigate their   respective antioxidant activities. Herein, an ultra-high pressure   liquid chromatography-mass spectrometer (UPLC-MS/MS) analytical   method has been developed for the separation, and systematic   characterization of the phenolic compounds in HS, CA and AV   extracts and was compared along with ten standard phenolic   compounds. Additionally, in vitro antioxidant activity of the   phenolic compounds was also determined. The HS extract revealed   excellent antioxidant activity such as   1,1-diphenyl-2-picrylhydrazyl radical scavenging activity   (IC50=19.7±1.2μg/mL), total reduction capability (0.169±0.003 at   100μg/mL), nitric oxide radical scavenging activity   (IC50=39.33±3.2μg/mL), metal chelating activity   (IC50=56.51±3.6μg/mL) and inhibition of lipid peroxidation   (IC50=12.34±2.3μg/mL) as compared to CA and AV extracts.   Furthermore, catechin, epicatechin, quercetin and chlorogenic   acid were found to be the major components responsible for the   antioxidant activity of the HS extract as evidenced from   UPLC-MS/MS. Taken together, this study demonstrates the promising   antioxidant properties of the HS extract, which can further be   utilized in various pharmaceutical, food, and agricultural   applications.

      Address (URL): http://www.ncbi.nlm.nih.gov/pubmed/26948646