Lee Schechtman - Formation of Short Chain Length/Medium Chain Length Polyhydroxyalkanoate Copolymers by Fatty Acid β-Oxidation Inhibited Ralstonia eutropha

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      Publication Details (including relevant citation   information):

      Green, Phillip R., Kemper, Joe, Schechtman, Lee, Guo, Ling,   Satkowski, Mike, Fiedler, Silke, Steinbüchel, Alexander, Rehm,   Bernd H. A. Biomacromolecules 1988  3 (1) 208-213

      Abstract: Ralstonia eutropha has been considered   as a bacterium, incorporating hydroxyalkanoates of less than six   carbons only into polyhydroxyalkanoates (PHAs). Cells of the wild   type cultivated with sodium octanoate as the carbon source in the   presence of the fatty acid β-oxidation inhibitor sodium acrylate   synthesized PHAs composed of the medium chain length   hydroxyalkanoates (3HAMCL) 3-hydroxyhexanoate (3HHx) and   3-hydroxyoctanoate (3HO) as well as of 3-hydroxybutyrate and   3-hydroxyproprionate as revealed by gas chromatography, 1H NMR   spectroscopy, and mass spectroscopy. The characterization of the   polymer as a tetrapolymer was confirmed by differential solvent   extraction and measurement of melting and glass transition   temperature depression in the purified polymer compared to PHB.   These data suggested that the R. eutropha PHA synthase is capable   of incorporating longer chain substrates than suggested by   previous in vitro studies. Furthermore, expression of the class   II PHA synthase gene phaC1 from P. aeruginosa in R. eutropha   resulted in the accumulation of PHAs consisting of 3HAMCL   contributing about 3−5% to cellular dry weight. These PHAs were   composed of nearly equal molar fractions of 3HO and   3-hydroxydecanoate (3HD) with traces of 3HHx. These data   indicated that 3HAMCL-CoA thioesters were diverted from the fatty   acid β-oxidation pathway towards PHA biosynthesis in recombinant   R. eutropha.

      Address (URL): http://dx.doi.org/10.1021/bm015620m