Elancheran Ramakrishnan - In vitro and In vivo Antioxidant, Anti-hyperlipidemic Properties and Chemical Characterization of Centella asiatica (L.) Extract

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      Publication Details (including relevant citation   information):

        Frontiers in Pharmacology

      Abstract:

        The study aimed to identify the phenolic compounds present in   Centella   asiatica (L.) (C.   asiatica) extract   and evaluate the respective antioxidant potential as well as   its cholesterol-lowering   effects in the experimental animal model. Herein, the   antioxidant potential of   extracts was assessed by its free radical scavenging activity   such as 2, 2-diphenyl -1-   picrylhydrazyl as well as reducing capability. The   anti-hyperlipidemic effects   of C.   asiatica extract   (CAE) were evaluated in high cholesterol-fed (HCF)   rats for 4 weeks, where   different concentrations of extracts (0.25, 0.5, and 1 g/kg/day)   were orally administrated   daily. Lipid and antioxidant profiles, including total   cholesterol (TC), triglyceride (TG), low density lipoprotein   cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and superoxide dismutase   (SOD), together with the indices of hepatic functions were also examined.   C.   asiatica revealed   excellent free radical scavenging activity as revealed by 2-2-   diphenyl-1-picryl-hydrazyl (DPPH) assay, with the IC50   values (9.62 ± 0.88   µg/mL).   Furthermore, C.   asiatica extracts   and fenofibrate remarkably   lowered the level of TC, TG, LDL-C, and showed elevated levels of   HDL-C, SOD. The histopathological   observations further demonstrated clear   differentiation and   structural changes in liver of HCF and CAE treated group.   Furthermore, gulonic acid,   ferulic acid, kaempferol, chlorogenic acid, and asiatic acid were   identified to be the major   components which might be responsible for the antioxidant   activity of the C.   asiatica extract as   evidenced from an ultra-high performance liquid   chromatography–mass spectrometer.   Taken together, these results signifies the excellent antioxidant   and anti-hyperlipidemic   properties of C.   asiatica leaf   extracts, which might be useful for the treatment of oxidative-stress related diseases   such as   hyperlipidemia.

      Address (URL): http://journal.frontiersin.org/article/10.3389/fphar.2016.00400/full