Steven Stellman - Assessment of chlorinated pesticides and polychlorinated biphenyls in adipose breast tissue using a supercritical fluid extraction method

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      Publication Details (including relevant citation   information):

      Djordjevic, M. V., Hoffmann, D., Fan, J., Prokopczyk, B., Citron,   M. L., Stellman, S. D. 15 (11) 2581-5-

      Abstract: A precise and highly reproducible   analytical method was developed for the assessment of   organochlorinated pesticide and polychlorinated biphenyl residues   in adipose tissue (> or = 50 mg). The method can be utilized   for epidemiological studies on the significance of these   environmental pollutants in the etiology of breast cancer.   Supercritical fluid extraction (SFE) with CO2 and modified CO2   (addition of 5% dichloromethane) is employed to remove incurred   pesticide residues from adipose tissues that have been surgically   removed from breast cancer patients and controls. An alumina   sorbent, placed in the extracting vessel together with a   specimen, removes the bulk of co-extracted lipids; a subsequent   purification of the SFE extracts by column chromatography on   alumina removes the remaining traces of lipids that would   interfere with the gas chromatographic analysis with electron   capture detection. The method was tested by analyzing a Certified   Reference Material 430 pork fat with known amounts of pesticide   residues that are commonly found in fat or in foods with a high   fat content. The recoveries of analytes ranged from 73.4% for   endrin to 115% for alpha-, beta- and gamma-hexachlorocyclohexane,   hexachlorobenzene and dieldrin, with standard deviations of 4-12%   for individual analytes. The analysis of adipose tissue for   organochlorinated compounds on the basis of this new method   suggested that the pesticide levels were higher in breast cancer   patients than in controls. However, the small number of samples   analyzed in this study (n = 5, both groups) precludes definitive   conclusions. The most abundant compounds in both cases and   controls were p, p-DDE (379 +/- 286 and 160 +/- 149 p.p.b.) and   PCB (223 +/- 145 and 124 +/- 65.7 p.p.b.), followed by the   termiticide chlordane residues oxychlordane and transnonachlor.

      Address (URL): http://www.ncbi.nlm.nih.gov/pubmed/7955109