Publication Details (including relevant citation information):
B nzli,Jean-Claude G. 2014 1st (4) 125-197
Abstract: Understanding the structure and functional properties of cells, organs, and living organisms is a key challenge in present-day biology and medicine, which stimulates the development of imaging techniques with high resolution and sensitivity. Luminescence imaging possesses these attributes in addition to featuring high penetration depth. The drawback of organic chromophores is their sensitivity to photobleaching, their small Stokes' shifts, and their short excited state lifetimes, which renders difficult separation of the probe signal from the autofluorescence background. Since the mid 1980's, lanthanide chelates have successfully replaced organic chromophores in luminescence immunoassays since they are not much sensitive to photobleaching, lend themselves to time-resolved detection, and have easily recognizable sharp emission lines; therefore both wavelength and time discrimination concur to isolate the probe signal from the background noise.