Jean-Claude Bunzli - Luminescence Bioimaging with Lanthanide Complexes

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  B nzli,Jean-Claude G. 2014 1st (4)   125-197

  Abstract: Understanding the structure and   functional properties of cells, organs, and living organisms is a   key challenge in present-day biology and medicine, which   stimulates the development of imaging techniques with high   resolution and sensitivity. Luminescence imaging possesses these   attributes in addition to featuring high penetration depth. The   drawback of organic chromophores is their sensitivity to   photobleaching, their small Stokes' shifts, and their short   excited state lifetimes, which renders difficult separation of   the probe signal from the autofluorescence background. Since the   mid 1980's, lanthanide chelates have successfully replaced   organic chromophores in luminescence immunoassays since they are   not much sensitive to photobleaching, lend themselves to   time-resolved detection, and have easily recognizable sharp   emission lines; therefore both wavelength and time discrimination   concur to isolate the probe signal from the background noise.

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