Jean-Claude Bunzli - Increasing the efficiency of lanthanide luminescent bioprobes: bioconjugated silica nanoparticles as markers for cancerous cells

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      Eliseeva,S.V., Song,B., Vandevyver,C.D.B., Chauvin,A.S.,   Wacker,J.B., Bunzli,J.C.G. New Journal of Chemistry  2010 34 (12) 2915-2921

      Abstract: The lanthanide binuclear helicate   [Ln(2)(L(C2))(3)] has been embedded into bare and   NH(2)-functionalized silica nanoparticles (NPs) using   water-in-oil microemulsion technique. TEM analysis reveals both   [Ln(2)(L(C2))(3)]@SiO(2) and [Ln(2)(L(C2))(3)]@SiO(2)/NH(2)   nanoparticles having a spherical morphology and being   monodispersed with an average size of 55 +/- 5 and 90 +/- 10 nm,   respectively. The energy of the ligand triplet state, similar to   21 800 cm(-1) ([Gd(2)(L(C2))(3)]@NP), does not change upon   incorporation into silica nanoparticles and is optimal for   sensitizing Eu(III) luminescence. As a consequence,   [Eu(2)(L(C2))(3)]@SiO(2) and [Eu(2)(L(C2))(3)]@SiO(2)/NH(2) NPs   display red emission due to characteristic (5)D(0) > (7)F(J)   (J = 0-4) transitions with absolute quantum yield reaching 28%   for the latter. NH(2)-functionalized NPs have then been   conjugated with avidin (NP-avidin) or goat anti-mouse IgG   antibody (NP-IgG) to test them as luminescent biomarkers.   Time-resolved microscopy of immunocytochemical assays involving   recognition of mucin-like proteins expressed on breast cancer   MCF-7 cells by the 5D10 monoclonal antibody confirms that the   NP-IgG bioprobe displays specific luminescent signal with   signal-to-noise ratio approximate to 20% higher than the one   obtained for the bioconjugate of molecular   [Eu(2)(L(C2(COOH)))(3)] with IgG. In addition, immunoassays using   a streptavidin-coated plate and the NP-IgG probe are able to   detect 15 ng mL(-1) of the biotinylated 5D10 antibody with a   signal-to-noise ratio of 100

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