Jean-Claude Bunzli - Selective Breast Cancer Cell Capture, Culture, and Immunocytochemical Analysis Using Self-Assembled Magnetic Bead Patterns in a Microfluidic Chip

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  Publication Details (including relevant citation   information):

  Sivagnanam,V., Song,B., Vandevyver,C., Bunzli,J.C.G., Gijs,M.A.M.   Langmuir 2010 26 (9) 6091-6096

  Abstract: Separation and subsequent culturing of   MCF-7 breast cancer cells on self-assembled protein-coated   magnetic beads in a microfluidic chip is demonstrated. The beads   were patterned in situ inside a sealed microfluidic channel using   magnetic-field-assisted electrostatic self-assembly. Hereafter,   they were grafted by exposure to a solution of 5D10 monoclonal   antibodies (mAb) and fibronectin (EN), with the first being used   for immunospecific cell capture and the latter being used for   cell adhesion and growth. A solution of target MCF-7 cells mixed   with Jurkat cells was brought inside the microchannel, leading,   to specific MCF-7 cell capture; the latter were then cultured and   evidenced by cell immuno-luminescence

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