Jean-Claude Bunzli - Time-resolved luminescence microscopy of bimetallic lanthanide helicates in living cells

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      Publication Details (including relevant citation   information):

      Song,B., Vandevyver,C.D.B., Chauvin,A.S., Bunzli,J.C.G.   Organic & Biomolecular Chemistry  2008 6 (22) 4125-4133

      Abstract: The cellular uptake mechanism and   intracellular distribution of emissive lanthanide helicates have   been elucidated by time-resolved luminescence microscopy (TRLM).   The helicates are non-cytotoxic and taken up by normal (HaCat)   and cancer (HeLa, MCF-7) cells by endocytosis and show a late   endosomal-lysosomal cellular distribution. The lysosomes   predominantly localize around the nucleus and co-localize with   the endoplasmatic reticulum. The egress is slow and limited,   around 30% after 24 h. The first bright luminescent images can be   observed with an external concentration gradient of 5 mu M of the   Eu(III) helicate [Q = 0.21, tau = 2.43 ms], compared to > 10   mM when using conventional luminescence microscopy. Furthermore,   multiplex labeling could be achieved with the Tb(III) [Q = 0.11,   tau = 0.65 ms], and Sm(III) [Q = 0.0038, tau = 0.030 ms]   analogues

      Address (URL): WOS:000261744700010