Hassen Jaafar

Emerging Serial Block Face SEM 3D-imaging (SBF-SEM 3D)

Blog Post created by Hassen Jaafar on Feb 16, 2018

Article Source: 3D Elemental Mapping in the ESEM - A Combination of Serial Block-face SEM and EDS


The combination of serial block-face scanning electron microscopy and energy dispersive x-ray spectroscopy (EDS) is demonstrated for 3D elemental analysis of materials, enabling investigations of large sample volumes compared to other tomographic techniques.

This method enables a time saving quantitative elemental reconstruction of large sample volumes compared to FIB or TEM and represents an enhancement of mere EDS mapping.

Recently, ultramicrotomy has also been utilised for 3D Elemental Mapping and to assist high resolution imaging in the scanning electron microscope (SEM); the generation of relatively flat specimens removes roughness effects from the secondary electron signal and improves the backscattered electron yield due to removal of an oxidised or carbon contaminated surface.



Highlights on SBF-SEM 3D-imaging :

3D imaging by serial block face scanning electron microscopy for materials science using ultramicrotomy

Three Dimensional Imaging of Light Metals Using Serial Block Face Scanning Electron Microscopy (SBFSEM)

3View serial block face imaging

Serial Block Face Scanning Electron Microscopy Demonstration