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Silencing RNA (siRNA) is short segments of RNA which bind to formed RNA and prevent specific genes from being expressed. This is a powerful tool in gene therapy however the siRNA is very delicate and susceptible to degradation. For this reason, it requires advanced delivery systems. Recently, researchers at Hoshi University and Osaka University (Japan) utilized poly-(α,β)-dl-aspartic acid (PolyVivo Cat# AO005) from PolySciTech (www.polyscitech.com) as part of their investigation into the biodistribution of siRNA drug-delivery systems in-vivo. This research holds promise to enable therapeutic effects of this technology. Read more: Hattori, Yoshiyuki, Ayako Nakamura, Shiori Hanaya, Yuta Miyanabe, Yuki Yoshiike, Takuto Kikuchi, Kei-ichi Ozaki, and Hiraku Onishi. "Effect of chondroitin sulfate on siRNA biodistribution and gene silencing effect in mice after injection of siRNA lipoplexes." Journal of Drug Delivery Science and Technology (2017). http://www.sciencedirect.com/science/article/pii/S1773224717305051


“Abstract: Previously, we found that intravenous injection of chondroitin sulfate (CS), followed by intravenous injection of siRNA/cationic liposome complexes (siRNA lipoplexes) could deliver siRNAs to the liver and suppress expression of target genes. Here, we examined the effect of injection order of CS and siRNA lipoplexes on the biodistribution of siRNA and gene silencing in the liver after sequential injection. When siRNA lipoplexes were injected intravenously into mice, the siRNA largely accumulated in the lungs. However, injection of siRNA lipoplexes, followed by injection of CS, reduced siRNA accumulation in the lungs and increased it in the liver. In addition, agglutinates of erythrocytes caused by the addition of siRNA lipoplexes were re-dispersed by the addition of CS, indicating that the agglutinates accumulating in the lungs by injection of siRNA lipoplexes were broken up by CS injection. However, injection of apolipoprotein B (ApoB) siRNA lipoplexes, followed by injection of CS did not suppress ApoB mRNA levels in the liver. From there results on sequential injection, the injection order of CS and siRNA lipoplexes was important for gene silencing effects in the liver, although the sequential injection could deliver siRNA efficiently into the liver regardless of the injection order of CS and siRNA lipoplexes. Keywords: siRNA delivery; Liposome; Chondroitin sulfate; Liver; Gene silencing”