John Garner

PLGA-PEG-PLGA from PolySciTech used in development of berberine nanoparticle for heart-disease therapy

Blog Post created by John Garner on Jun 22, 2018

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Berberine is a historical herbal remedy garnering new attention over its capability to reduce cholesterol and provide for improved heart health. Due to its low bioavailability, its uptake and efficacy however can be improved by applying nanotechnology. Recently, researchers at University of Massachusetts Lowell used PLGA-PEG-PLGA (PolyVivo AK072) from PolySciTech (www.polyscitech.com) to generate nanoparticles loaded with berberine for improved oral uptake. This research holds promise to provide for improved therapies for high cholesterol and heart disease. Read more: Ochin, Chinedu C., and Mahdi Garelnabi. "Berberine Encapsulated PLGA-PEG Nanoparticles Modulate PCSK-9 in HepG2 Cells." Cardiovascular & Haematological Disorders-Drug Targets (Formerly Current Drug Targets-Cardiovascular & Hematological Disorders) 18, no. 1 (2018): 61-70. https://www.ingentaconnect.com/contentone/ben/chddt/2018/00000018/00000001/art00 010

“Background: The developments of new parenteral approaches to target PCSK-9 for the treatment of LDL-Cholesterol has yielded impressive results; and have shown significant decreases in the risk of mortality associated with hypercholesterolemia. However improved and convenient alternate approaches that exploit the beneficial drug target properties of PCSK-9 also need to be explored and developed. One such approach is the oral administration of Berberine using nanotechnology.  Methods: Nanoprecipitation encapsulation and physiochemical characterization of Berberine Chloride in PLGA-PEG-PLGA block copolymer has been developed and characterized in Hep-G2 cells using Berberine Chloride encapsulated nanoparticle (Bc-NP). Evaluation of PCSK-9, SREBP- 1, LDL-r, HNF-1alpha mRNAs and PCSK-9 protein expression was performed using quantitative real-time PCR (qPCR) and median fluorescence intensity (MFI) of flow cytometric studies respectively. Pearson's correlation analysis of PCSK-9 mRNA and protein levels in Berberine chloride delivery was performed.  Results: The PCSK-9 mRNA and protein expression shows a relationship to the release of Berberine from the encapsulating PLGA-PEG-PLGA polymer in a time dependent manner.  Conclusion: PCSK-9 modulation by oral administration of Berberine using nanotechnology approach can improve its pharmacokinetic profile. Further studies are needed to maximize its delivery efficiency. Keywords: PCSK9; atherosclerosis; drug delivery; lipids; lipoproteins; nanotechnology”

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