John Garner

PLGA-PEG-COOH from PolySciTech used in development of nucleolin-targeting nanoparticles

Blog Post created by John Garner on Oct 17, 2018

Mosafer, 2018 AS1411 PLGA-PEG polyscitech cancer.png

Often, in cancer, nucleolin is overexpressed on the surface which allows it to be used as a target for drug-delivery. Recently, researchers at Tabriz University of Medical Sciences (Iran) utilized PLGA-PEG-COOH (PolyVivo AI076) from PolySciTech (www.polyscitech.com) to generate anti-nucleolin decorated nanoparticles for cancer targeting. This research holds promise for improved chemotherapeutics. Read more: Mosafer, Jafar, and Ahad Mokhtarzadeh. "Cell Surface Nucleolin as a Promising Receptor for Effective AS1411 Aptamer-Mediated Targeted Drug Delivery into Cancer Cells." Current drug delivery 15, no. 9 (2018): 1323-1329. https://www.ingentaconnect.com/contentone/ben/cdd/2018/00000015/00000009/art0001 3

  “Background: One of the major abundant proteins in the nucleous is nucleolin that overexpressed on the cytoplasmic membrane of malignant and endothelial cells and makes it as a promising condidate for targeted drug delivery. Objectives: In this study, doxorubicin (Dox) as a chemotherapy drug was entrapped into the Poly lacticco- glycolic acid (PLGA)-based nanoparticles (NPs). Then, the targeting ability of anti nucleolin AS1411 aptamer-targeted Dox-encapsulated PLGA-based NPs (AS1411-NPs) was investigated in high nucleolin-expressing C26 colon carcinoma and rat C6 glioma cell lines compared with low nucleolin expressing mouse L929 cell line. Methods: We recently first assessed the existence of cell surface nucleolin of these three different cell lines by immunocytochemistry method. We found that a large amount of nucleolin was localized in the cytoplasmic membrane of C26 and C6 cell lines, with a very smaller amount on the surface of L929 cell line. Results: As a result, more rapidly internalization of AS1411-NPs into the C26 and C6 cells compared with L929 cells was verified. Conclusion: We think that AS1411-NPs, as a ligand, first bind to nucleolin, as a receptor, and then the receptor-ligand complex is more efficiently incorporated into the high nucleolin-expressing cell lines through receptor-mediated endocytosis pathway. Keywords: AS1411 aptamer; Nucleolin; PLGA; doxorubicin; internalization; targeted delivery”

Outcomes