John Garner

PLGA, PEG-PLGA, PLGA-Folate from PolySciTech used in research on chemotherapeutic nanocarriers

Blog Post created by John Garner on Aug 19, 2019

There are a multitude of nanoparticle strategies currently in development for applications towards treatment of cancer. Despite this, there is still a great deal which remains to be discovered in terms of the exact nature of the nanoparticle/biological interactions. Additionally, the optimal formulation approach remains to be determined. Recently, researchers at Wroclaw University (Poland) used PLGA (AP022), mPEG-PLGA (AK037), and PLGA-Folate (AO037) from PolySciTech (www.polyscitech.com) to develop different types of nanoparticles and tested their use for chemotherapy delivery. This research holds promise for improved therapies against cancer in the future. Read more: Bazylińska, Urszula, Julita Kulbacka, and Grzegorz Chodaczek. "Nanoemulsion Structural Design in Co-Encapsulation of Hybrid Multifunctional Agents: Influence of the Smart PLGA Polymers on the Nanosystem-Enhanced Delivery and Electro-Photodynamic Treatment." Pharmaceutics 11, no. 8 (2019): 405.  https://www.mdpi.com/1999-4923/11/8/405

“Abstract: In the present study, we examined properties of poly(lactide-co-glycolide) (PLGA)-based nanocarriers (NCs) with various functional or “smart” properties, i.e., coated with PLGA, polyethylene glycolated PLGA (PEG-PLGA), or folic acid-functionalized PLGA (FA-PLGA). NCs were obtained by double emulsion (water-in-oil-in-water) evaporation process, which is one of the most suitable approaches in nanoemulsion structural design. Nanoemulsion surface engineering allowed us to co-encapsulate a hydrophobic porphyrin photosensitizing dye—verteporfin (VP) in combination with low-dose cisplatin (CisPt)—a hydrophilic cytostatic drug. The composition was tested as a multifunctional and synergistic hybrid agent for bioimaging and anticancer treatment assisted by electroporation on human ovarian cancer SKOV-3 and control hamster ovarian fibroblastoid CHO-K1 cell lines. The diameter of PLGA NCs with different coatings was on average 200 nm, as shown by dynamic light scattering, transmission electron microscopy, and atomic force microscopy. We analyzed the effect of the nanocarrier charge and the polymeric shield variation on the colloidal stability using microelectrophoretic and turbidimetric methods. The cellular internalization and anticancer activity following the electro-photodynamic treatment (EP-PDT) were assessed with confocal microscopy and flow cytometry. Our data show that functionalized PLGA NCs are biocompatible and enable efficient delivery of the hybrid cargo to cancer cells, followed by enhanced killing of cells when supported by EP-PDT. Keywords: smart nanocarriers; folic acid; verteporfin; cisplatin; SKOV-3 cells; CHO-K1 cells; electroporation; theranostic cargo; double emulsion approach”

Biotech, Pharma, Cancer, Research (BPCR) is a free, 1-day scientific networking conference hosted by Akina, Inc. on Aug 28, 2019. See more and register at http://bpcrconference.com

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