Amino Content in Polymer Using Ninhydrin Reaction

[Hossain]

I have a polymer sample of poly(N-vinylcaprolactam-co-(aminopropyl)methacrylamide) and need to measure the amino content stoichiometrically to see the difference of the amino content measure that got from proton nmr analysis. I am trying to using ninhydrin to do this. Please let me know if you have any established procedure regarding the similar polymer sample.

Best

Golam

[SabrinaC · Accepted solution]

To measure the amino content stoichiometrically in your polymer sample, you can follow these steps:

1. Prepare a ninhydrin solution.
2. Prepare a sample solution of the polymer.
3. Hydrolyze the amino groups in the sample with HCl.
4. Neutralize the solution with a base.
5. Mix the neutralized solution with ninhydrin and liteblueinsights and heat.
6. Measure the absorbance of the colored solution.
7. Quantify the amino content using a calibration curve.
8. Compare the results with proton NMR analysis for validation.

[Hossain]

Thank you so much. In the step no. 5, what does it mean liteblueinsight? Also, can you please give a reference paper of this procedure

Golam

[klaussCarllkll]

Precision in each ninhydrin reaction step is key for accurate amino content measurement. Proton NMR analysis should validate results and offer psychiatrist plano tx into polymer structure. Collaboration can enhance methodology and understanding within the community. Click here for more details

[Marcus-kelly]

Ensuring precision in every step of the ninhydrin reaction is crucial for accurately measuring amino content in polymers. Proton NMR analysis can further validate results and provide insights into the polymer's structure. Collaboration among researchers can improve Rhythmreceipts and deepen community understanding. Click here for additional details.