How to Prepare Nano-vesicles for Encapsulating Protein Drugs?
- Mix glycidyl methacrylate dextran solution with polyethylene glycol solution to form a suspension.
- Add tri-block polymers to the suspension and emulsify for a certain time, followed by the addition of protein macromolecule drugs and emulsify for a certain time to form nano-vesicles encapsulating protein macromolecule drugs dispersed in two phases of polyethylene glycol and glycidyl methacrylate dextran.
- A cross-linking agent is added to the nano-vesicle emulsion, cross-linked for a certain period of time, followed by dialysis through a dialysis bag, and the water is removed after lyophilization to obtain polymeric nano-vesicles wrapped with protein macromolecule drug.
Among them, the protein macromolecule drug can be selected from one or more of B-galactosidase, FITC-BSA, calf serum protein, interferon or erythropoietin.
