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122287
New Contributor

Determination of Quantum Yield

Is there a relatively easy experimental method to determine the quantum yield of a fluorophore in different buffer solutions?  Thanks

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3 Replies
ahmadtuhl
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Re: Determination of Quantum Yield

Hi I am also working to determination of quantum yield of fluorescence ,triplet stat and singlet oxygen

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jkunjappu
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Re: Determination of Quantum Yield

Identify a water soluble dye like Rhodamines or so that can work in buffers, I guess; determine its exact quantum yield first,  and compare with the system in question.  The absorption band of the dye and the system molecule should match.  Corrections for concentration and relative absorbance are essential.

JTK

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MFWahab
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Re: Determination of Quantum Yield

A quite simple experimental set up is described in

http://pubs.acs.org/doi/abs/10.1021/ed076p1260

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