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hdiaz
New Contributor

Natural Products Isolation

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What do you think is better for isolating compounds from aqueous extracts of plants? TLC, Liquid-Liquid extraction, HPLC or Countercurrent Chromatography?

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kparunscientist
New Contributor

Re: Natural Products Isolation

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Hi,

I think U should go step by step, Start with Liquid-liquid extraction then TLC confirmation, then Column chromatography followed by Detection of compounds through HPLC MS / GC MS / NMR these techniques are based on ur compounds of interest.

Arun KP

Do anyone help me in finding the Database for LC MS/MS pattern on Natural products (Free Online)???

Thanks

Arun KP

View solution in original post

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May_
New Contributor II

Re: Natural Products Isolation

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I think it depends on the compounds your trying to isolate. we, in my University, usually use HPLC.

good luck

rlagoa
New Contributor II

Re: Natural Products Isolation

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You have to decide according to physical-chemical properties of the compounds to isolate, such as polarity (eventually pH-dependant). The compounds to isolate are known or to be identified? If they are known, the properties can be predicted or, even better, procedures may already be available in the literature. The volume of the extracts can also be determinant to choose isolation technique. Take in attention if your compounds are heat temperature-sensitive.

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hdiaz
New Contributor

Re: Natural Products Isolation

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Thanks Ricardo. The compounds are to be identified, and the quantity of the extract will be aprox 1gr to isolate the compouds in it.

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kparunscientist
New Contributor

Re: Natural Products Isolation

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Hi,

I think U should go step by step, Start with Liquid-liquid extraction then TLC confirmation, then Column chromatography followed by Detection of compounds through HPLC MS / GC MS / NMR these techniques are based on ur compounds of interest.

Arun KP

Do anyone help me in finding the Database for LC MS/MS pattern on Natural products (Free Online)???

Thanks

Arun KP

View solution in original post

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