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Substituting anhydrous for monohydrous and vice versa

Question asked by Drew Opland-Evers on Nov 14, 2018
Latest reply on Nov 15, 2018 by Thomas Flynn


I'm preparing a growth medium for mold, following the recipe below.


Dissolve the following ingredients, in the order listed, into 500ml of cold tap water: 44.0 grams Lactose Monohydrate, 25.0 grams cornstarch, 3.0 grams sodium nitrate, 0.25 grams magnesium sulfate, 0.50 grams potassium phosphate mono, 2.75 grams glucose monohydrate, 0.044 grams zinc sulfate, 0.044 grams manganese sulfate. Then add enough cold tap water to make one liter. Use hydrochloric acid to adjust the pH to between 5.0 and 5.5.


But, We're short on chemicals. We don't have lactose monohydrate, but we have lactose (anhydrous). We don't have glucose monohydrate, but we have dextrose anhydrate. We don't have anhydrous zinc sulfate, but we have zinc sulfate (monohydrous). Is it possible to substitute these chemicals in with the ones we have on hand? Is there a way to convert the chemicals on hand to their monohydrous/anhydrous form?