Preparation of Peptide-oligonucleotide Conjugation
There are several different strategies for synthesizing peptide and oligonucleotide conjugates, including solid phase synthesis, liquid phase synthesis, and biosynthesis. When the peptide is conjugated to the oligonucleotide, the peptide is capable of binding to the base of the oligonucleotide, the 3' or 5' end of the ribose or the phosphate backbone. Likewise, the point of attachment of the oligonucleotide and peptide can be the C-terminus, N-terminus or amino acid side chain of the peptide.
