Is there a relatively easy experimental method to determine the quantum yield of a fluorophore in different buffer solutions? Thanks
Hi I am also working to determination of quantum yield of fluorescence ,triplet stat and singlet oxygen
Identify a water soluble dye like Rhodamines or so that can work in buffers, I guess; determine its exact quantum yield first, and compare with the system in question. The absorption band of the dye and the system molecule should match. Corrections for concentration and relative absorbance are essential.
A quite simple experimental set up is described in
Retrieving data ...