Does hydrogen bonding of acrylamide with another molecule affect its absolute contribution in UV absorbance?
in detail if X concentration of acrylamide has AX absorbance at 200 nm, by adding another molecule which not absorb at this wavelength and interact with acrylamide only by hydrogen bonding, does the absorbance value (AX) affected? Why ? if yes, increases or decreases? why?
(the UV absorbance measured around 200 nm because of C=O bonds)
Thanks in advance for your prompt response