Samantha Gildenhuys

Mass spec users-HELP!

Discussion created by Samantha Gildenhuys on Mar 28, 2013
Latest reply on Mar 29, 2013 by Darryl Davis

I hope that I can get some advice from Mass spec users. I am currently looking at purchasing a MASS spec instrument with LC separation, that can conduct HDX-MS experiments as well as general protein identification and post translational modifications. Then, and this is my first question, Can an instrument specialised for this type of protein work also be used for vitamin and amino acid identification?

What would my best choice of LC be? UPLC nano, or capillary or just normal HPLC and then also should I have a sort of 2D set up?

Then ion source suppliers have been competing some say MALDI others say ESI, which is best?

Finally the actual MS instrument which is the leader in this type of work? I need to hear which ones work best form users to guide my decision.

Would love to hear feedback, I am rather clueless never worked on MS before setting up whole new lab.

Samantha in South Africa