Hi, I'm looking for help to process a DOSY NMR spectrum. I'd like to know how to transform the gradient decay plot for a given region of the spectrum into a plot showing the diffusion constants (x-axis) vs the intensity (y-axis), sort of a distribution of diffusion constants. I've read about it online, and this website Diffusion NMR suggests applying an inverse laplace transform to a gaussian fitting function for the gradient decay plot, but I'm not sure how to do this. I was hoping someone could show me the steps to obtain this plot.

Thank you for your time.

Jorge,

As with most NMR data, the transformation is usually accomplished either within the software provided by the spectrometer vendor or by third-party software such as MNova from Mestrelab. I also think that these vendors use some proprietary routines for getting a good 2D plot (for example, minimizing the "streaking" one encounters when two peaks are close in chemical shift but have different diffusion constants).

Is there a particular reason you want to do this manually? Do you have access to vendor-supplied software?

Thanks. - Josh