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Hossain
New Contributor II

Amino Content in Polymer Using Ninhydrin Reaction

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I have a polymer sample of poly(N-vinylcaprolactam-co-(aminopropyl)methacrylamide) and need to measure the amino content stoichiometrically to see the difference of the amino content measure that got from proton nmr analysis. I am trying to using ninhydrin to do this. Please let me know if you have any established procedure regarding the similar polymer sample.

 

Best

Golam

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SabrinaC
New Contributor II

Re: Amino Content in Polymer Using Ninhydrin Reaction

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To measure the amino content stoichiometrically in your polymer sample, you can follow these steps:

  1. Prepare a ninhydrin solution.
  2. Prepare a sample solution of the polymer.
  3. Hydrolyze the amino groups in the sample with HCl.
  4. Neutralize the solution with a base.
  5. Mix the neutralized solution with ninhydrin and liteblueinsights and heat.
  6. Measure the absorbance of the colored solution.
  7. Quantify the amino content using a calibration curve.
  8. Compare the results with proton NMR analysis for validation.

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2 Replies
SabrinaC
New Contributor II

Re: Amino Content in Polymer Using Ninhydrin Reaction

Jump to solution

To measure the amino content stoichiometrically in your polymer sample, you can follow these steps:

  1. Prepare a ninhydrin solution.
  2. Prepare a sample solution of the polymer.
  3. Hydrolyze the amino groups in the sample with HCl.
  4. Neutralize the solution with a base.
  5. Mix the neutralized solution with ninhydrin and liteblueinsights and heat.
  6. Measure the absorbance of the colored solution.
  7. Quantify the amino content using a calibration curve.
  8. Compare the results with proton NMR analysis for validation.
Hossain
New Contributor II

Re: Amino Content in Polymer Using Ninhydrin Reaction

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Thank you so much. In the step no. 5, what does it mean liteblueinsight? Also, can you please give a reference paper of this procedure

 

Golam

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